Plasmodium falciparum nucleoplasmin (PfNPM)

Ketul Saharan.

SASDVR2 – Nucleoplasmin-like domain-containing protein PfNPM (1-110)

Nucleoplasmin-like domain-containing protein A1
MWexperimental 67 kDa
MWexpected 67 kDa
VPorod 141 nm3
log I(s) 2.43×102 2.43×101 2.43×100 2.43×10-1
Nucleoplasmin-like domain-containing protein A1 small angle scattering data  s, nm-1
ln I(s)
Nucleoplasmin-like domain-containing protein A1 Guinier plot ln 2.43×102 Rg: 2.8 nm 0 (2.8 nm)-2 s2
(sRg)2I(s)/I(0)
Nucleoplasmin-like domain-containing protein A1 Kratky plot 1.104 0 √3 sRg
p(r)
Nucleoplasmin-like domain-containing protein A1 pair distance distribution function Rg: 2.9 nm 0 Dmax: 8.6 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Nucleoplasmin-like domain-containing protein A1 DAMMIF model
log I(s)
 s, nm-1
Nucleoplasmin-like domain-containing protein A1 ALPHAFOLD model
Synchrotron SAXS data from solutions of Nucleoplasmin-like domain-containing protein PfNPM (1-110) in 20 mM Tris, 150 mM NaCl, 2 mM β-mercaptoethanol, pH 7.5 were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.09794 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 20°C. 10 successive 20 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Nucleoplasmin-like domain-containing protein A1 (PfNPM A1)
Mol. type   Protein
Organism   Plasmodium falciparum 3D7
Olig. state   Pentamer
Mon. MW   13.4 kDa
 
UniProt   C0H4U4 (1-110)
Sequence   FASTA