Plasmodium falciparum nucleoplasmin (PfNPM)

Ketul Saharan.

SASDVS2 – Nucleoplasmin-like domain-containing protein PfNPM (1-170)

N-terminal domain of Nucleoplasmin-like domain-containing protein A3
MWexperimental 102 kDa
MWexpected 103 kDa
VPorod 204 nm3
log I(s) 3.66×102 3.66×101 3.66×100 3.66×10-1
N-terminal domain of Nucleoplasmin-like domain-containing protein A3 small angle scattering data  s, nm-1
ln I(s)
N-terminal domain of Nucleoplasmin-like domain-containing protein A3 Guinier plot ln 3.67×102 Rg: 4.2 nm 0 (4.2 nm)-2 s2
(sRg)2I(s)/I(0)
N-terminal domain of Nucleoplasmin-like domain-containing protein A3 Kratky plot 1.104 0 √3 sRg
p(r)
N-terminal domain of Nucleoplasmin-like domain-containing protein A3 pair distance distribution function Rg: 4.4 nm 0 Dmax: 14.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
N-terminal domain of Nucleoplasmin-like domain-containing protein A3 DAMMIF model
log I(s)
 s, nm-1
N-terminal domain of Nucleoplasmin-like domain-containing protein A3 ALPHAFOLD model
Synchrotron SAXS data from solutions of Nucleoplasmin-like domain-containing protein PfNPM (1-170) in 20 mM Tris, 150 mM NaCl, 2 mM β-mercaptoethanol, pH 7.5 were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.09794 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 20°C. 10 successive 20 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

N-terminal domain of Nucleoplasmin-like domain-containing protein A3 (PfNPM A3)
Mol. type   Protein
Organism   Plasmodium falciparum 3D7
Olig. state   Pentamer
Mon. MW   20.5 kDa
 
UniProt   C0H4U4 (1-170)
Sequence   FASTA