Peripheral membrane protein endophilin B1 probes, perturbs and permeabilizes lipid bilayers

Thorlacius A, Rulev M, Sundberg O, Sundborger-Lunna A, Communications Biology 8(1) (2025) DOI

SASDVT4 – Endophilin B1 wild type (0.14 mg/ml)

Endophilin-B1
MWexperimental 37 kDa
MWexpected 41 kDa
VPorod 47 nm3
log I(s) 4.30×101 4.30×100 4.30×10-1 4.30×10-2
Endophilin-B1 small angle scattering data  s, nm-1
ln I(s)
Endophilin-B1 Guinier plot ln 4.30×101 Rg: 3.7 nm 0 (3.7 nm)-2 s2
(sRg)2I(s)/I(0)
Endophilin-B1 Kratky plot 1.104 0 √3 sRg
p(r)
Endophilin-B1 pair distance distribution function Rg: 4.3 nm 0 Dmax: 15.3 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Synchrotron SAXS data from solutions of endophilin B1 in 20 mM HEPES, 150 mM NaCl, 1 mM TCEP, 0.5 mM DTT, pH 8.1 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.09918 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.69 mg/ml was measured at 4°C. 15 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Endophilin-B1
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   40.8 kDa
 
UniProt   Q9Y371 (1-365)
Sequence   FASTA