Synchrotron SAXS data from solutions of CELSR1 ECR with calcium in 10 mM Tris, 150 mM NaCl, 1 mM CaCl2, pH 8.5 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory storage ring (Lemont, IL, USA) using a Eiger2 XE 9M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 250.00 μl sample at 1.0 mg/ml was injected at a 0.60 ml/min flow rate onto a GE Superose 6 Increase 10/300 column at 22°C. 2500 successive 0.400 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
s, nm-1
