| MWexperimental | 151 | kDa |
| MWexpected | 156 | kDa |
| VPorod | 284 | nm3 |
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log I(s)
2.80×101
2.80×100
2.80×10-1
2.80×10-2
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s, nm-1
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Plasmodium falciparum nucleoplasmin (PfNPM)
Ketul Saharan.SASDVU2 – Nucleoplasmin-like domain-containing protein PfNPM (1-170) - H3/H4 histone oligomer complex
Histone H3.1PfNPM A3 (1-170)
Histone H4
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Fits and models
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Synchrotron SAXS
data from solutions of
Nucleoplasmin-like domain-containing protein PfNPM (1-170) - H3/H4 histone oligomer complex
in
20 mM Tris, 150 mM NaCl, 2 mM β-mercaptoethanol, pH 7.5
were collected
on the
BM29 beam line
at the ESRF storage ring
(Grenoble, France)
using a Pilatus3 2M detector
at a sample-detector distance of 2.9 m and
at a wavelength of λ = 0.09794 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 1.00 mg/ml was measured
at 20°C.
10 successive
20 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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