Synchrotron SAXS data from solutions of nsp14 and nsp10 from SARS-CoV-2 with vhh in 25 mM HEPES, 300 mM NaCl, 5 mM CaCl2, pH 8 were collected on the SWING beam line at SOLEIL (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.103317 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 17.4 mg/ml was injected at a 0.30 ml/min flow rate onto a Agilent Bio SEC-3, 300 Å column at 20°C. 800 successive 0.990 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
For display purposes, the primary SAXS data of this entry has been scaled by 10000. The original unscaled data are made available in the full entry zip archive.
s, nm-1
