| MWI(0) | 76 | kDa |
| MWexpected | 74 | kDa |
| VPorod | 110 | nm3 |
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log I(s)
3.48×10-2
3.48×10-3
3.48×10-4
3.48×10-5
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s, nm-1
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A SEC-SAXS analysis of SARS-CoV-2 nsp14 apo or in complex with vhh and/or nsp10
Pierre Gauffre.SASDVX8 – nsp14 from SARS-CoV-2 with vhh
Replicase polyprotein 1ab (Guanine-N7 methyltransferase nsp14)vhh anti-nsp14 SARS-CoV-2
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Fits and models
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Synchrotron SAXS data from solutions of nsp14 from SARS-CoV-2 with vhh in 25 mM HEPES, 300 mM NaCl, 5 mM CaCl2, pH 8 were collected on the SWING beam line at SOLEIL (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.103317 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 90.00 μl sample at 8.5 mg/ml was injected at a 0.30 ml/min flow rate onto a Agilent Bio SEC-3, 300 Å column at 20°C. 800 successive 0.990 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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