| MWI(0) | 75 | kDa |
| MWexpected | 78 | kDa |
| VPorod | 142 | nm3 |
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log I(s)
2.48×10-2
2.48×10-3
2.48×10-4
2.48×10-5
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s, nm-1
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Cryo-EM led analysis of open and closed conformations of Chagas vaccine candidate TcPOP.
Batra S, Olmo F, Ragan TJ, Kaplan M, Calvaresi V, Frank AM, Lancey C, Assadipapari M, Ying C, Struwe WB, Hesketh EL, Kelly JM, Barfod L, Campeotto I, Nat Commun 16(1):7164 (2025) Europe PMCSASDW23 – SEC-SAXS of Trypanosoma cruzi Prolyl Oligopepetidase (TcPOP)
Prolyl endopeptidase|
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Fits and models
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Synchrotron SAXS
data from solutions of
SEC-SAXS of Trypanosoma cruzi Prolyl Oligopepetidase (TcPOP)
in
20 mM HEPES, 150 mM NaCl, pH 7.4
were collected
on the
B21 beam line
at the Diamond Light Source storage ring
(Didcot, UK)
using a Eiger 4M detector
at a sample-detector distance of 3.7 m and
at a wavelength of λ = 0.09464 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample
at 8 mg/ml was injected at a 0.08 ml/min flow rate
onto a GE Superdex 200 Increase 5/150 column
at 15°C.
600 successive
3 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
CAUTION! Primary display data is different to the data used for modelling! CAUTION! Data used for modelling has NO GUINIER REGION, i.e., does not encompass the particle size/volume and radius of gyration! CAUTION! The models displayed in this entry were NOT used to calculate the fits to the data and therefore have no connection/relevance to the fits displayed in this entry (interpret model fits with extreme caution)! |
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