Batra S,
Olmo F,
Ragan TJ,
Kaplan M,
Calvaresi V,
Frank AM,
Lancey C,
Assadipapari M,
Ying C,
Struwe WB,
Hesketh EL,
Kelly JM,
Barfod L,
Campeotto I,
Nat Commun
16(1):7164
(2025)
Europe PMC
SASDW33 – STATIC-SAXS of Trypanosoma cruzi Prolyl Oligopepetidase (TcPOP)
Synchrotron SAXS
data from solutions of
STATIC-SAXS of Trypanosoma cruzi Prolyl Oligopepetidase (TcPOP)
in
20 mM HEPES, 150 mM NaCl, pH 7.4
were collected
on the
B21 beam line
at the Diamond Light Source storage ring
(Didcot, UK)
using a Eiger 4M detector
at a sample-detector distance of 3.7 m and
at a wavelength of λ = 0.09464 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 0.3 and 6 mg/ml were measured
at 15°C.
21 successive
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.
CAUTION! Data are severely over subtracted. CAUTION! Severe aggregation. CAUTION! The Rg, I(0), particle size/volume, molecular weight, p(r) and any other structural parameters derived from the data are undefined/uninterpretable.
s, nm-1
