Teneurin-3 isoforms, WT and R2579W

Maja Napieraj.

SASDWA8 – Teneurin-3 wildtype A1B0 isoform in 2 mM calcium buffer - 0.23 mg/ml

Isoform A1B0 of Teneurin-3 wildtype
MWexperimental 647 kDa
MWexpected 535 kDa
VPorod 1222 nm3
log I(s) 1.51×102 1.51×101 1.51×100 1.51×10-1
Isoform A1B0 of Teneurin-3 wildtype small angle scattering data  s, nm-1
ln I(s)
Isoform A1B0 of Teneurin-3 wildtype Guinier plot ln 1.51×102 Rg: 8.6 nm 0 (8.6 nm)-2 s2
(sRg)2I(s)/I(0)
Isoform A1B0 of Teneurin-3 wildtype Kratky plot 1.104 0 √3 sRg
p(r)
Isoform A1B0 of Teneurin-3 wildtype pair distance distribution function Rg: 8.8 nm 0 Dmax: 36.9 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Teneurin-3 wildtype A1B0 isoform in 2 mM calcium buffer - 0.23 mg/ml in 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH 7.8 were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.0992 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.23 mg/ml was measured at 20°C. 10 successive 0.200 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Isoform A1B0 of Teneurin-3 wildtype (Ten3-A1B0 WT)
Mol. type   Protein
Organism   Mus musculus
Olig. state   Dimer
Mon. MW   267.5 kDa
 
UniProt   Q9WTS6-4 (343-2708)
Sequence   FASTA