Biophysical analysis of an oligomerization-attenuated variant of the Leishmania donovani dynamin-1-like protein.

Wuyts E, Sundaramoorthy R, Tulloch L, Monsieurs P, Eadsforth TC, Pintelon I, Timmermans JP, Dujardin JC, Domagalska MA, Caljon G, De Rycker M, Postis VLG, Wyllie S, Sterckx YG, Mol Biochem Parasitol 263:111691 (2025) Europe PMC

SASDWB7 – Dynamin central region family protein from Leishmania donovani DLP1 G354D / R357S

Dynamin central region family protein
MWexperimental 164 kDa
MWexpected 165 kDa
VPorod 340 nm3
log I(s) 2.89×10-2 2.89×10-3 2.89×10-4 2.89×10-5
Dynamin central region family protein small angle scattering data  s, nm-1
ln I(s)
Dynamin central region family protein Guinier plot ln 2.89×10-2 Rg: 6.6 nm 0 (6.6 nm)-2 s2
(sRg)2I(s)/I(0)
Dynamin central region family protein Kratky plot 1.104 0 √3 sRg
p(r)
Dynamin central region family protein pair distance distribution function Rg: 7 nm 0 Dmax: 25.0 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Dynamin central region family protein BILBOMD model
log I(s)
 s, nm-1
Synchrotron SAXS data from solutions of Dynamin central region family protein from Leishmania donovani DLP1 G354D / R357S in 20 mM HEPES, 200 mM NaCl, 3% glycerol, pH 7.4 were collected on the SWING beam line at the SOLEIL storage ring (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 6.44 mg/ml was measured at 20°C. 6848 successive 0.750 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Dynamin central region family protein
Mol. type   Protein
Organism   Leishmania donovani
Olig. state   Dimer
Mon. MW   82.4 kDa
 
UniProt   E9BL28
Sequence   FASTA