| MWexperimental | 53 | kDa |
| MWexpected | 63 | kDa |
| VPorod | 84 | nm3 |
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log I(s)
6.66×104
6.66×103
6.66×102
6.66×101
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s, nm-1
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SAXS analysis of EccA5 N-terminal domain and EspG5 of ESX-5 secretion pathway of Mycobacterium tuberculosis
Rajlakshmi K.SASDWF7 – EccA5-N terminal domain and EspG5 complex of ESX-5 secretion pathway of Mycobacterium tuberculosis
ESX-5 secretion-associated protein EspG5ESX-5 secretion system protein EccA5 N-terminal domain
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Fits and models
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SAXS
data from solutions of
EccA5-N terminal domain and EspG5 complex of ESX-5 secretion pathway of Mycobacterium tuberculosis
in
50mM Hepes 200mM NaCl, pH 8
were collected
on the
Anton Paar SAXSpace instrument (CSIR-Central Drug Research Institute, Lucknow, India)
using a Mythen2 R 1K detector
at a sample-detector distance of 0.3 m and
at a wavelength of λ = 0.154 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 4.00 mg/ml was measured
at 10°C.
Four successive
900 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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