Teneurin-3 isoforms, WT and R2579W

Maja Napieraj.

SASDWT7 – Teneurin-3 mutant A0B1 isoform in 2 mM calcium buffer - 1.05 mg/ml

Isoform A0B1 of Teneurin-3 mutant
MWexperimental 873 kDa
MWexpected 535 kDa
VPorod 1336 nm3
log I(s) 6.54×102 6.54×101 6.54×100 6.54×10-1
Isoform A0B1 of Teneurin-3 mutant small angle scattering data  s, nm-1
ln I(s)
Isoform A0B1 of Teneurin-3 mutant Guinier plot ln 6.54×102 Rg: 8.4 nm 0 (8.4 nm)-2 s2
(sRg)2I(s)/I(0)
Isoform A0B1 of Teneurin-3 mutant Kratky plot 1.104 0 √3 sRg
p(r)
Isoform A0B1 of Teneurin-3 mutant pair distance distribution function Rg: 8.8 nm 0 Dmax: 32.8 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Teneurin-3 mutant A0B1 isoform in 2 mM calcium buffer - 1.05 mg/ml in 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH 7.8 were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.0992 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.2 and 1.1 mg/ml were measured at 20°C. 10 successive 0.200 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Isoform A0B1 of Teneurin-3 mutant (Ten3-A0B1 R2579W)
Mol. type   Protein
Organism   Mus musculus
Olig. state   Dimer
Mon. MW   267.4 kDa
 
UniProt   Q9WTS6-3 (343-2706)
Sequence   FASTA