Teneurin-3 isoforms, WT and R2579W

Maja Napieraj.

SASDWV7 – Teneurin-3 mutant A1B0 isoform in 2 mM calcium buffer - 1.03 mg/ml

Isoform A1B0 of Teneurin-3 R2579W
MWexperimental 585 kDa
MWexpected 535 kDa
VPorod 1236 nm3
log I(s) 6.37×102 6.37×101 6.37×100 6.37×10-1
Isoform A1B0 of Teneurin-3 R2579W small angle scattering data  s, nm-1
ln I(s)
Isoform A1B0 of Teneurin-3 R2579W Guinier plot ln 6.38×102 Rg: 8.2 nm 0 (8.2 nm)-2 s2
(sRg)2I(s)/I(0)
Isoform A1B0 of Teneurin-3 R2579W Kratky plot 1.104 0 √3 sRg
p(r)
Isoform A1B0 of Teneurin-3 R2579W pair distance distribution function Rg: 8.8 nm 0 Dmax: 35.4 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Teneurin-3 mutant A1B0 isoform in 2 mM calcium buffer - 1.03 mg/ml in 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH 7.8 were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.0992 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.2 and 1.0 mg/ml were measured at 20°C. 10 successive 0.200 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Isoform A1B0 of Teneurin-3 R2579W (Ten3-A1B0 R2579W)
Mol. type   Protein
Organism   Mus musculus
Olig. state   Dimer
Mon. MW   267.5 kDa
 
UniProt   Q9WTS6-4 (343-2708)
Sequence   FASTA