Teneurin-3 isoforms, WT and R2579W

Maja Napieraj.

SASDWW7 – Teneurin-3 wildtype A1B1 isoform in 2 mM calcium buffer - 0.93 mg/ml

Isoform A1B1 of Teneurin-3 wildtype
MWexperimental 585 kDa
MWexpected 537 kDa
VPorod 1210 nm3
log I(s) 6.00×102 6.00×101 6.00×100 6.00×10-1
Isoform A1B1 of Teneurin-3 wildtype small angle scattering data  s, nm-1
ln I(s)
Isoform A1B1 of Teneurin-3 wildtype Guinier plot ln 6.00×102 Rg: 8.1 nm 0 (8.1 nm)-2 s2
(sRg)2I(s)/I(0)
Isoform A1B1 of Teneurin-3 wildtype Kratky plot 1.104 0 √3 sRg
p(r)
Isoform A1B1 of Teneurin-3 wildtype pair distance distribution function Rg: 8.5 nm 0 Dmax: 31.6 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Teneurin-3 wildtype A1B1 isoform in 2 mM calcium buffer - 0.93 mg/ml in 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH 7.8 were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.0992 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.93 mg/ml was measured at 20°C. 10 successive 0.200 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Isoform A1B1 of Teneurin-3 wildtype (Ten3-A1B1 protein WT)
Mol. type   Protein
Organism   Mus musculus
Olig. state   Dimer
Mon. MW   268.4 kDa
 
UniProt   Q9WTS6-1 (343-2715)
Sequence   FASTA