SASDWZ9 – Small EDRK-rich factor 1 protein (SERF1a) at pH 6.8

Isoform Short of Small EDRK-rich factor 1

MW^experimental	10	kDa
MW^expected	7	kDa
V^Porod	11	nm³

log I(s) 1.16×10^-2 1.16×10^-3 1.16×10^-4 1.16×10^-5

Isoform Short of Small EDRK-rich factor 1 small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

Isoform Short of Small EDRK-rich factor 1 Guinier plot

ln 1.16×10^-2 R_g: 2.5 nm 0 (2.5 nm)^-2 s²

(sR_g)²I(s)/I(0)

Isoform Short of Small EDRK-rich factor 1 Kratky plot

1.104 0 √3 sR_g

p(r)	R_g: 2.6 nm 0 D_max: 8.8 nm

Data validation

Experimental data range

p(r) fit to data

Metric

Value

s_min D_max / π

0.3

N_Shannon

Worse

Better

Metric

Value

p_cormap

χ²

0.002
0.583

Worse

Better

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Isoform Short of Small EDRK-rich factor 1 OTHER model

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Synchrotron SAXS data from solutions of SERF1a 20 mM sodium phosphate, 20 mM NaCl, pH 6.8 were collected on the 13A beam line at the Taiwan Photon Source, NSRRC (Hsinchu, Taiwan) using a Eiger X 9M detector at a sample-detector distance of 2.5 m and at a wavelength of λ = 0.08266 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 15.00 mg/ml was measured at 10°C. Five successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Isoform Short of Small EDRK-rich factor 1 (SERF1a)
Mol. type		Protein
Organism		Homo sapiens
Olig. state		Monomer
Mon. MW		7.3 kDa

UniProt		O75920-2 (1-62)
Sequence		FASTA