| MWexperimental | 234 | kDa |
| MWexpected | 205 | kDa |
| VPorod | 400 | nm3 |
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log I(s)
7.76×10-1
7.76×10-2
7.76×10-3
7.76×10-4
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s, nm-1
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Structure of the chromoprotein ovorubin from the golden apple snail ( Pomacea canaliculata )
Wilasluck P, Saw W, Tran B, Sabat G, Shih O, Hengphasatporn K, Shigeta Y, Vinayavekhin N, Wangkanont K, Protein Science 35(1) (2025) DOISASDX24 – Ovorubin from the golden apple snail (Pomacea canaliculata)
Perivitellin ovorubin-1Perivitellin ovorubin-2
Uncharacterized protein (ovorubin-3, short)
Perivitellin protein (ovorubin-4, form 1)
Uncharacterized protein (ovorubin-5)
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Fits and models
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Synchrotron SAXS data from solutions of ovorubin from the golden apple snail in 20 mM Tris, 150 mM NaCl, pH 7 were collected on the TPS13A beam line at the NSRRC (Hsinchu, Taiwan) using a Eiger X 1M & 9M detector at a sample-detector distances of 0.75 m and 5.5 m and at a wavelength of λ = 0.082656 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 10 mg/ml was injected at a 0.35 ml/min flow rate onto a Agilent Bio SEC-3, 300 Å column at 25°C. Five successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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