| MWexperimental | 146 | kDa |
| MWexpected | 146 | kDa |
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log I(s)
6.43×101
6.43×100
6.43×10-1
6.43×10-2
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s, nm-1
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Crystal structure of a complete threonyl tRNA synthetase: toggle clamp model?
Tran T, Fenwick M, Edwards T, Dranow D, Abendroth J, Shek R, Tillery L, Craig J, Van Voorhis W, Myler P, Biochemical and Biophysical Research Communications :152677 (2025) DOISASDX28 – SmThrRS model for closed state based on PDB entry 1qf6
Stenotrophomonas maltophilia threonyl tRNA synthetase|
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Fits and models
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Synchrotron SAXS
data from solutions of
SmThrRS model for closed state based on PDB entry 1qf6
in
20 mM HEPES, 0.2 M NaCl, 1.5% v/v glycerol, and 1 mM TCEP, pH 7
were collected
on the
12.3.1 (SIBYLS) beam line
at the Advanced Light Source (ALS) storage ring
(Berkeley, CA, USA)
using a Pilatus3 X 2M detector
at a sample-detector distance of 2.1 m and
at a wavelength of λ = 0.1127 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 3.10 mg/ml was measured
at 20°C.
660 successive
2 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The trace was processed with Chromixs. The IFT was computed in BioXtas-RAW 2.3.0 using DENSS. The Dmax was set to 162 Angstroms. GNOM gives a similar Dmax at a qmax of 0.3 A^-1. |
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