| MWexperimental | 207 | kDa |
| MWexpected | 197 | kDa |
| VPorod | 483 | nm3 |
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log I(s)
8.14×10-2
8.14×10-3
8.14×10-4
8.14×10-5
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s, nm-1
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Trypanosome histone variants H3.V and H4.V promote nucleosome plasticity in repressed chromatin
SASDX93 – T. brucei H3.V+H4.V Nucleosome Core Particle
Histone H2AHistone H2B
Widom 601 145 bp DNA - strand 1
Widom 601 145 bp DNA - strand 2
Histone H3 variant
Histone H4
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There are no models related to this curve.
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Synchrotron SAXS
data from solutions of
T. brucei H3.V+H4.V Nucleosome Core Particle
in
20 mM HEPES, 150 mM NaCl, 1 mM DTT, pH 7.5
were collected
on the
B21 beam line
at the Diamond Light Source storage ring
(Didcot, UK)
using a Eiger 4M detector
at a sample-detector distance of 3.7 m and
at a wavelength of λ = 0.09464 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample
at 2 mg/ml was injected at a 0.08 ml/min flow rate
onto a GE Superdex 200 Increase 3.2/300 column
at 15°C.
600 successive
3 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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