Non Structural Protein Sars CoV-2

Hira Singh Gariya.

SASDXB6 – Non Structural Protein 15 (Replicase polyprotein 1ab) from SARS Cov-2

Non structural Protein 15
MWI(0) 208 kDa
MWexpected 228 kDa
VPorod 392 nm3
log I(s) 7.02×104 7.02×103 7.02×102 7.02×101
Non structural Protein 15 small angle scattering data  s, nm-1
ln I(s)
Non structural Protein 15 Guinier plot ln 7.03×104 Rg: 4.5 nm 0 (4.5 nm)-2 s2
(sRg)2I(s)/I(0)
Non structural Protein 15 Kratky plot 1.104 0 √3 sRg
p(r)
Non structural Protein 15 pair distance distribution function Rg: 4.4 nm 0 Dmax: 13.7 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Non structural Protein 15 PDB (PROTEIN DATA BANK) model
SAXS data from solutions of Non Structural Protein 15 (Replicase polyprotein 1ab) from SARS Cov-2 in 20mM HEPES, 150mM Nacl, pH 7.5 were collected on the Anton Paar SAXSpace instrument (CSIR-Central Drug Research Institute, Lucknow, India) using a Mythen2 R 1K detector at a sample-detector distance of 0.3 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 7.00 mg/ml was measured at 10°C. Three successive 900 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Non structural Protein 15 (NSP15)
Mol. type   Protein
Organism   Severe acute respiratory syndrome coronavirus 2
Olig. state   Hexamer
Mon. MW   38.0 kDa
 
UniProt   P0DTD1 (6465-6798)
Sequence   FASTA
 
PDB ID   7N33