| MWexperimental | 56 | kDa |
| MWexpected | 56 | kDa |
| VPorod | 84 | nm3 |
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log I(s)
3.72×101
3.72×100
3.72×10-1
3.72×10-2
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s, nm-1
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Characterisation of Entamoeba histolytica Anti-Silencing Function 1 as a histone chaperone.
Gandhi S, Vasudevan D, Biochimie (2025) Europe PMCSASDXC7 – Entamoeba histolytica Anti-Silencing Function 1 (ASF1)_Histone H3-H4 dimer complex
Anti-Silencing Function 1Histone H3
Histone H4
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Fits and models
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Synchrotron SAXS
data from solutions of
Entamoeba histolytica Anti-Silencing Function 1 (ASF1)_Histone H3-H4 dimer complex
in
20 mM Tris, 150 mM NaCl, and 2 mM β-mercaptoethanol, pH 7.5
were collected
on the
BM29 beam line
at the ESRF storage ring
(Grenoble, France)
using a Pilatus3 2M detector
at a sample-detector distance of 2.9 m and
at a wavelength of λ = 0.9794 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 1.16 mg/ml was measured
at 20°C.
10 successive
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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