Dimers of full-length SFPQ observed by SANS

Heidar Koning.

SASDXD4 – Full-length human SFPQ dimer (protiated and deuterated)

Splicing factor, proline- and glutamine-rich
MWexperimental 197 kDa
MWexpected 153 kDa
VPorod 241000 nm3
log I(s) 2.38×10-1 2.38×10-2 2.38×10-3 2.38×10-4
Splicing factor, proline- and glutamine-rich small angle scattering data  s, nm-1
ln I(s)
Splicing factor, proline- and glutamine-rich Guinier plot ln 2.38×10-1 Rg: 8.7 nm 0 (8.7 nm)-2 s2
(sRg)2I(s)/I(0)
Splicing factor, proline- and glutamine-rich Kratky plot 1.104 0 √3 sRg
p(r)
Splicing factor, proline- and glutamine-rich pair distance distribution function Rg: 8.6 nm 0 Dmax: 30.7 nm

Data validation

There are no models related to this curve.

SANS data from solutions of Full-length human SFPQ dimer (protiated and deuterated) in 150 mM KCl, 1.5% glycerol, 20 mM HEPES, 1 mM DTT (in 100% H2O), pH 7.4 were collected using a ORDELA/21000N detector at a sample-detector distance of 1.3 m and at a wavelength of λ = 0.6 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.63 mg/ml was measured at 25°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

An experiment on a mixture of protiated (0.03 mg/ml) and deuterated (0.6 mg/ml) dimers of full-length SFPQ. 600 uL of sample was in 150 mM KCl, 1.5% glycerol, 20 mM HEPES, 1 mM DTT, pH 7.4 (100% H2O) and scattering was collected at QUOKKA ANSTO Lucas Heights. This data is part of the manuscript 'Structural dynamics of IDR interactions in human SFPQ and implications for liquid-liquid phase separation'

Splicing factor, proline- and glutamine-rich (SFPQ cysteine dimer)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   76.3 kDa
 
UniProt   P23246 (214-707)
Sequence   FASTA