Structural dynamics of IDR interactions in human SFPQ and implications for liquid–liquid phase separation

Koning H, Lai V, Sethi A, Chakraborty S, Ang C, Fox A, Duff A, Whitten A, Marshall A, Bond C, Acta Crystallographica Section D Structural Biology 81(7):357-379 (2025) DOI

SASDXD4 – Full-length human SFPQ dimer (protiated and deuterated)

Splicing factor, proline- and glutamine-rich
MWexperimental 197 kDa
MWexpected 153 kDa
VPorod 241000 nm3
log I(s) 2.38×10-1 2.38×10-2 2.38×10-3 2.38×10-4
Splicing factor, proline- and glutamine-rich small angle scattering data  s, nm-1
ln I(s)
Splicing factor, proline- and glutamine-rich Guinier plot ln 2.38×10-1 Rg: 8.7 nm 0 (8.7 nm)-2 s2
(sRg)2I(s)/I(0)
Splicing factor, proline- and glutamine-rich Kratky plot 1.104 0 3 sRg
p(r)
Splicing factor, proline- and glutamine-rich pair distance distribution function Rg: 8.6 nm 0 Dmax: 30.7 nm

Data validation


There are no models related to this curve.

SANS data from solutions of Full-length human SFPQ dimer (protiated and deuterated) in 150 mM KCl, 1.5% glycerol, 20 mM HEPES, 1 mM DTT (in 100% H2O), pH 7.4 were collected using a ORDELA/21000N detector at a sample-detector distance of 1.3 m and at a wavelength of λ = 0.6 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.63 mg/ml was measured at 25°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

An experiment on a mixture of protiated (0.03 mg/ml) and deuterated (0.6 mg/ml) dimers of full-length SFPQ. 600 uL of sample was in 150 mM KCl, 1.5% glycerol, 20 mM HEPES, 1 mM DTT, pH 7.4 (100% H2O) and scattering was collected at QUOKKA ANSTO Lucas Heights. This data is part of the manuscript 'Structural dynamics of IDR interactions in human SFPQ and implications for liquid-liquid phase separation'

Splicing factor, proline- and glutamine-rich (SFPQ cysteine dimer)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   76.3 kDa
 
UniProt   P23246 (214-707)
Sequence   FASTA