Solution structure of human MALAT1-associated small cytoplasmic RNA (mascRNA)

Claude Sauter.

SASDXD9 – MALAT1-associated small cytoplasmic RNA (mascRNA)

MALAT1-associated small cytoplasmic RNA
MWexperimental 18 kDa
MWexpected 20 kDa
VPorod 22 nm3
log I(s) 2.80×10-2 2.80×10-3 2.80×10-4 2.80×10-5
MALAT1-associated small cytoplasmic RNA small angle scattering data  s, nm-1
ln I(s)
MALAT1-associated small cytoplasmic RNA Guinier plot ln 2.81×10-2 Rg: 2.0 nm 0 (2.0 nm)-2 s2
(sRg)2I(s)/I(0)
MALAT1-associated small cytoplasmic RNA Kratky plot 1.104 0 √3 sRg
p(r)
MALAT1-associated small cytoplasmic RNA pair distance distribution function Rg: 2.1 nm 0 Dmax: 7.2 nm

Data validation

Fits and models

log I(s)
 s, nm-1
MALAT1-associated small cytoplasmic RNA ROSETTA model
Synchrotron SAXS data from solutions of MALAT1-associated small cytoplasmic RNA (mascRNA) in 50 mM HEPES-NaOH, 150 NaCl, 10 mM MgCl2, pH 7.5 were collected on the SWING beam line at the SOLEIL storage ring (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 40.00 μl sample at 2.8 mg/ml was injected at a 0.30 ml/min flow rate onto a Agilent Bio SEC-3, 300 Å column at 25°C. 660 successive 990 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

MALAT1-associated small cytoplasmic RNA (mascRNA)
Mol. type   RNA
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   19.6 kDa
Sequence   FASTA