| MWexperimental | 88 | kDa |
| MWexpected | 92 | kDa |
| VPorod | 141 | nm3 |
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log I(s)
1.61×104
1.61×103
1.61×102
1.61×101
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s, nm-1
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Small angle x-ray scattering analysis of PE25, PPE41, EspG5 and EccA5 N-terminal domain complex of ESX-5 pathway of Mycobacterium tuberculosis
Rajlakshmi K.SASDXE7 – Complex of PE25, PPE41, EspG5 and EccA5 N-terminal domain of ESX-5 pathway of Mycobacterium tuberculosis
PE-PGRS family protein PE25PPE family protein PPE41
ESX-5 secretion-associated protein EspG5
ESX-5 secretion system protein EccA5
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Fits and models
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SAXS
data from solutions of
Complex of PE25, PPE41, EspG5 and EccA5 N-terminal domain of ESX-5 pathway of Mycobacterium tuberculosis
in
50mM HEPES, 200mM NaCl, pH 8
were collected
on the
Anton Paar SAXSpace instrument (CSIR-Central Drug Research Institute, Lucknow, India)
using a Mythen2 R 1K detector
at a sample-detector distance of 0.3 m and
at a wavelength of λ = 0.154 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 3.00 mg/ml was measured
at 10°C.
Four successive
900 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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