A new type of flexible CP12 protein in the marine diatom Thalassiosira pseudonana

Shao H, Huang W, Avilan L, Receveur-Bréchot V, Puppo C, Puppo R, Lebrun R, Gontero B, Launay H, Cell Communication and Signaling 19(1) (2021) DOI

SASDXG3 – CP12 protein from the marine diatom Thalassiosira pseudonana

CP12 domain-containing protein
MWI(0) 42 kDa
MWexpected 34 kDa
log I(s) 5.36×10-2 5.36×10-3 5.36×10-4 5.36×10-5
CP12 domain-containing protein small angle scattering data  s, nm-1
ln I(s)
CP12 domain-containing protein Guinier plot ln 5.36×10-2 Rg: 3.8 nm 0 (3.8 nm)-2 s2
(sRg)2I(s)/I(0)
CP12 domain-containing protein Kratky plot 1.104 0 √3 sRg
Dmax: 13.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
CP12 domain-containing protein GASBOR model
Synchrotron SAXS data from solutions of CP12 protein from the marine diatom Thalassiosira pseudonana in 30 mM Tris, 50 mM NaCl, 2 mM EDTA, 1 mM TCEP, pH 7.5 were collected on the SWING beam line at the SOLEIL storage ring (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 11 mg/ml was injected at a 0.30 ml/min flow rate onto a Agilent Bio SEC-3, 300 Å column at 15°C. 400 successive 0.990 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Protein with His-Tag

CP12 domain-containing protein (CP12)
Mol. type   Protein
Organism   Thalassiosira pseudonana
Olig. state   Dimer
Mon. MW   17.2 kDa
 
UniProt   B8BQS1
Sequence   FASTA