Synchrotron SAXS
data from solutions of
poly(ADP-ribose)polymerase 1 (Af-PARP1) 5'P nicked DNA complex
in
20 mM HEPES, 150 mM NaCl, 2 mM TCEP, 2.5% (v/v) Glycerol, pH 7.5
were collected
on the
B21 beam line
at the Diamond Light Source storage ring
(Didcot, UK)
using a Eiger 4M detector
at a sample-detector distance of 3.7 m and
at a wavelength of λ = 0.09464 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 4.60 mg/ml was measured
at 15°C.
600 successive
3 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
s, nm-1
