| MWexperimental | 58 | kDa |
| MWexpected | 68 | kDa |
| VPorod | 70 | nm3 |
|
log I(s)
3.29×10-3
3.29×10-4
3.29×10-5
3.29×10-6
|
s, nm-1
|
Hexahistidine-tagged QseC bound to operator DNA sites
Joshua Ramsay.SASDXJ8 – Hexahistidine-tagged Quorum sensing controller protein (QseC) bound to dual operator OL and OR DNA sites
Quorum sensing controller protein QseCQseC Operator OL+OR DNA
|
|
|
Fits and models
|
|
|
Synchrotron SAXS
data from solutions of
Hexahistidine-tagged Quorum sensing controller protein (QseC) bound to dual operator OL and OR DNA sites
in
50mM Tris, 300mM NaCl, 2.5% glycerol, pH 7.4
were collected
on the
SAXS/WAXS beam line
at the Australian Synchrotron storage ring
(Melbourne, Australia)
using a Pilatus 1M detector
at a sample-detector distance of 1.6 m and
at a wavelength of λ = 0.10332 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 60.00 μl sample
at 3.5 mg/ml was injected at a 0.30 ml/min flow rate
onto a GE Superdex 200 Increase 5/150 column
at 25°C.
800 successive
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
|
|
|||||||||||||||||||||||||||||||||||||||||||||