SAXS
data from solutions of
Histone deacetylase 5 (HDAC5)
in
25 mM HEPES, 150 mM KCl, 1 mM DTT,, pH 7.5
were collected
on the
Xenocs Xeuss 2.0 Q-Xoom instrument (Center for Structural Studies, Heinrich-Heine-University, Düsseldorf, Germany)
using a Pilatus3 R 300K detector
at a sample-detector distance of 0.6 m and
at a wavelength of λ = 0.154 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 2.15 mg/ml was measured
at 15°C.
24 successive
600 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
s, nm-1
