| MWI(0) | 26659 | kDa |
| MWexpected | 159 | kDa |
| VPorod | 213 | nm3 |
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log I(s)
2.69×105
2.69×104
2.69×103
2.69×102
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s, nm-1
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RdRp
SASDXW5 – Replicase polyprotein 1ab-ORF1ab polyprotein (RdRp) protein complex from SARS CoV-2
Replicase polyprotein 1abReplicase polyprotein 1ab
ORF1ab polyprotein
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Fits and models
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SAXS
data from solutions of
Replicase polyprotein 1ab-ORF1ab polyprotein (RdRp) protein complex from SARS CoV-2
in
50 mM HEPES, 200 mM NaCl, pH 8
were collected
on the
Anton Paar SAXSpace instrument (CSIR-Central Drug Research Institute, Lucknow, India)
using a Mythen2 R 1K detector
at a sample-detector distance of 0.3 m and
at a wavelength of λ = 0.154 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 7.20 mg/ml was measured
at 10°C.
Four successive
900 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Rdrp of SARS CoV-2. The first eight (8) data points were removed from the original dataset. The original scattering data file is included as an additional attachment. |
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