SAXS
data from solutions of
Angiotensin converting Enzyme 2 bound to Receptor binding domain
in
50mM Tris, 200mM NaCl, pH 8
were collected
on the
Anton Paar SAXSpace instrument (CSIR-Central Drug Research Institute, Lucknow, India)
using a Mythen2 R 1K detector
at a sample-detector distance of 0.3 m and
at a wavelength of λ = 0.154 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 0.65 mg/ml was measured
at 10°C.
Two successive
1800 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The ACE2 RBD complex was incubated with Umifenovir compound for approximately 30 minutes before the SAXS analysis.
s, nm-1
