The phytoplasma effector, phyllogen, structurally mimics host plant MADS transcription factors.

Galien A, Hutin S, Le Hir S, Paul M, Albanese P, Kieffer-Jaquinod S, Guillotin A, Iwabuchi N, Maejima K, Nanao MH, Tully MD Hugouvieux V, Zubieta C, J Biol Chem 302(10):113449 (2026) Europe PMC

SASDY37 – MADS transcription factor SEPALLATA 3 (SEP3)

Developmental protein SEPALLATA 3
MWexperimental 55 kDa
MWexpected 24 kDa
VPorod 530 nm3
log I(s) 1.62×10-1 1.62×10-2 1.62×10-3 1.62×10-4
Developmental protein SEPALLATA 3 small angle scattering data  s, nm-1
ln I(s)
Developmental protein SEPALLATA 3 Guinier plot ln 1.63×10-1 Rg: 4.0 nm 0 (4.0 nm)-2 s2
(sRg)2I(s)/I(0)
Developmental protein SEPALLATA 3 Kratky plot 1.104 0 √3 sRg
p(r)
Developmental protein SEPALLATA 3 pair distance distribution function Rg: 4.0 nm 0 Dmax: 15.4 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of MADS transcription factor SEPALLATA 3 (SEP3) in 50 mM Tris, 300 mM NaCl, 1 mM TCEP, pH 8 were collected on the B21 beam line at the Diamond Light Source storage ring (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 11.30 mg/ml was measured at 20°C. 620 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Collected via SEC-SAXS. Flexible molecule.

Developmental protein SEPALLATA 3 (SEP3)
Mol. type   Protein
Organism   Arabidopsis thaliana
Olig. state   Dimer
Mon. MW   11.8 kDa
 
UniProt   O22456 (75-178)
Sequence   FASTA