Mpxv DNA Ligase A50

Anke Chen.

SASDY46 – Monkeypox virus (Mpxv) DNA Ligase A50

Mpxv DNA Ligase A50
MWexperimental 65 kDa
MWexpected 64 kDa
VPorod 116 nm3
log I(s) 1.71×101 1.71×100 1.71×10-1 1.71×10-2
Mpxv DNA Ligase A50 small angle scattering data  s, nm-1
ln I(s)
Mpxv DNA Ligase A50 Guinier plot ln 1.71×101 Rg: 4.0 nm 0 (4.0 nm)-2 s2
(sRg)2I(s)/I(0)
Mpxv DNA Ligase A50 Kratky plot 1.104 0 √3 sRg
p(r)
Mpxv DNA Ligase A50 pair distance distribution function Rg: 4.2 nm 0 Dmax: 17.9 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Mpxv DNA Ligase A50 DAMMIN model
Synchrotron SAXS data from solutions of Monkeypox virus (Mpxv) DNA Ligase A50 in 50 mM MES, 300 mM NaCl, pH 6.5 were collected on the BL19U2 beam line at the Shanghai Synchrotron Radiation Facility (SSRF) storage ring (Shanghai, China) using a Pilatus 1M detector at a sample-detector distance of 1 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 25°C. One 1 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Mpxv DNA Ligase A50 (MpxvLIG A50)
Mol. type   Protein
Organism   Monkeypox virus (strain Zaire-96-I-16) (MPX)
Olig. state   Monomer
Mon. MW   64 kDa
 
UniProt   Q8V4S9 (1-554)
Sequence   FASTA