Advances on the large-scale bacterial production and stable phosphorylation of Hsc70

Kley N, Hecht-Bucher M, Itzen A, Protein Expression and Purification :106923 (2026) DOI

SASDYB5 – Phosphorylated (T495) Heat shock cognate 71 kDa protein (Hsc70) T204A with ATP (4 mg/ml)

Phosphorylated Heat shock cognate 71 kDa protein
MWexperimental 86 kDa
MWexpected 71 kDa
VPorod 149 nm3
log I(s) 6.79×10-2 6.79×10-3 6.79×10-4 6.79×10-5
Phosphorylated Heat shock cognate 71 kDa protein small angle scattering data  s, nm-1
ln I(s)
Phosphorylated Heat shock cognate 71 kDa protein Guinier plot ln 6.80×10-2 Rg: 4.3 nm 0 (4.3 nm)-2 s2
(sRg)2I(s)/I(0)
Phosphorylated Heat shock cognate 71 kDa protein Kratky plot 1.104 0 √3 sRg
p(r)
Phosphorylated Heat shock cognate 71 kDa protein pair distance distribution function Rg: 4.2 nm 0 Dmax: 16.7 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Phosphorylated (T495) Heat shock cognate 71 kDa protein (Hsc70) T204A with ATP (4 mg/ml) in 20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1 mM TCEP, 1 mM ATP, pH 7.5 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.123979 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.00 mg/ml was measured at 20°C. 40 successive 0.095 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Phosphorylated Heat shock cognate 71 kDa protein (pHsc70)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Other
Mon. MW   71.1 kDa
 
UniProt   P11142 (1-646)
Sequence   FASTA