ACE 2 RBD

Raja Tripathi.

SASDYG7 – Angiotensin-converting Enzyme 2 with Spike glycoprotein Receptor binding domain, along with Umifenovir compound

Angiotensin-converting enzyme 2
Spike glycoprotein
MWexperimental 66 kDa
MWexpected 705 kDa
log I(s) 2.21×104 2.21×103 2.21×102 2.21×101
Angiotensin-converting enzyme 2 Spike glycoprotein small angle scattering data  s, nm-1
ln I(s)
Angiotensin-converting enzyme 2 Spike glycoprotein Guinier plot ln 2.21×104 Rg: 4.4 nm 0 (4.4 nm)-2 s2
(sRg)2I(s)/I(0)
Angiotensin-converting enzyme 2 Spike glycoprotein Kratky plot 1.104 0 3 sRg
p(r)
Angiotensin-converting enzyme 2 Spike glycoprotein pair distance distribution function Rg: 4.2 nm 0 Dmax: 11.4 nm

Data validation


There are no models related to this curve.

SAXS data from solutions of Angiotensin-converting Enzyme 2 with Spike glycoprotein Receptor binding domain, along with Umifenovir compound in 50mM Tris, 150mM NaCl, pH 8 were collected on the Anton Paar SAXSpace instrument (CSIR-Central Drug Research Institute, Lucknow, India) using a Mythen2 R 1K detector at a sample-detector distance of 0.3 m and at a wavelength of λ = 0.1514 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.66 mg/ml was measured at 10°C. Two successive 1800 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

ACE2 RBD complex.

Angiotensin-converting enzyme 2 (ACE 2)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Hexamer
Mon. MW   92.5 kDa
 
UniProt   Q9BYF1 (1-805)
Sequence   FASTA
 
Spike glycoprotein (RBD)
Mol. type   Protein
Organism   Severe acute respiratory syndrome coronavirus 2
Olig. state   Hexamer
Mon. MW   25 kDa
 
UniProt   P0DTC2 (319-541)
Sequence   FASTA