RnaseA

Anthony Giannetti.

SASDYH2 – RnaseA at pH 7.4

Bovine Ribonuclease pancreatic
MWI(0) 16 kDa
MWexpected 14 kDa
VPorod 16 nm3
log I(s) 1.13×102 1.13×101 1.13×100 1.13×10-1
Bovine Ribonuclease pancreatic small angle scattering data  s, nm-1
ln I(s)
Bovine Ribonuclease pancreatic Guinier plot ln 1.13×102 Rg: 1.6 nm 0 (1.6 nm)-2 s2
(sRg)2I(s)/I(0)
Bovine Ribonuclease pancreatic Kratky plot 1.104 0 √3 sRg
p(r)
Bovine Ribonuclease pancreatic pair distance distribution function Rg: 1.6 nm 0 Dmax: 60 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Bovine Ribonuclease pancreatic DAMMIF model
log I(s)
 s, nm-1
Synchrotron SAXS data from solutions of RnaseA at pH 7.4 in 20 mM HEPES, 150 mM NaCl, pH 7.4 were collected on the BL4-2 beam line at the Stanford Synchrotron Radiation Lightsource (SSRL) storage ring (Menlo Park, CA, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 1.2 m and at a wavelength of λ = 0.1127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 10.00 mg/ml was measured at 15°C. 16 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Bovine Ribonuclease pancreatic (RnaseA)
Mol. type   Protein
Organism   Bos taurus
Olig. state   Monomer
Mon. MW   13.7 kDa
 
UniProt   P61823 (27-150)
Sequence   FASTA