The Distinct Structural Propensities of Poly-C, A, and U Single-Stranded RNA

Wang T He W, Fronhofer S, Kirmizialtin S, Pollack L, Biomacromolecules (2026) DOI

SASDYH4 – Poly-cytosine rC30 RNA in 100 mM NaCl

30mer of poly-cytidine RNA
MWexperimental 10 kDa
MWexpected 9 kDa
log I(s) 3.44×10-2 3.44×10-3 3.44×10-4 3.44×10-5
30mer of poly-cytidine RNA small angle scattering data  s, nm-1
ln I(s)
30mer of poly-cytidine RNA Guinier plot ln 3.45×10-2 Rg: 2.6 nm 0 (2.6 nm)-2 s2
(sRg)2I(s)/I(0)
30mer of poly-cytidine RNA Kratky plot 1.104 0 √3 sRg

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Poly-cytosine rC30 RNA in 100 mM NaCl in 100 mM NaCl, 1 mM MOPS, 20 µM EDTA, pH 7.1 were collected on the ID7A1 BioSAXS / HP-Bio Beamline beam line at the Cornell High Energy Synchrotron Source (CHESS) storage ring (Ithaca, NY, USA) using a Eiger 4M detector at a sample-detector distance of 1.8 m and at a wavelength of λ = 0.1094 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.90 mg/ml was measured at 25°C. 100 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

30mer of poly-cytidine RNA
Mol. type   RNA
Olig. state   Monomer
Mon. MW   9.1 kDa
Sequence   FASTA