Interpreting wide-angle X-ray scattering data using DNA duplexes as model systems.

Hu Q Amirbekian V, Uttormark S, Higinbotham HR, Pollack L, IUCrJ 13(Pt 5):650-658 (2026) Europe PMC

SASDYJ9 – SWAXS from AT DNA duplex (25 base pair)

AT-sequence 25 base-paired DNA
MWexperimental 13 kDa
MWexpected 16 kDa
log I(s) 3.54×10-2 3.54×10-3 3.54×10-4 3.54×10-5
AT-sequence 25 base-paired DNA small angle scattering data  s, nm-1
ln I(s)
AT-sequence 25 base-paired DNA Guinier plot ln 3.54×10-2 Rg: 2.2 nm 0 (2.2 nm)-2 s2
(sRg)2I(s)/I(0)
AT-sequence 25 base-paired DNA Kratky plot 1.104 0 √3 sRg
p(r)
AT-sequence 25 base-paired DNA pair distance distribution function Rg: 2.3 nm 0 Dmax: 8.5 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of SWAXS from AT DNA duplex (25 base pair) in 10 mM MOPS, 150 mM NaCl, 50 µM EDTA, pH 7 were collected on the 16-ID (LiX) beam line at the National Synchrotron Light Source II (NSLS-II) storage ring (Upton, NY, USA) using a Pilatus 1M detector at a wavelength of λ = 0.0819 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). at 25°C. 10 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Sample detector distance = UNKNOWN. Concentration max = UNKNOWN

AT-sequence 25 base-paired DNA (AT DNA)
Mol. type   DNA
Olig. state   Dimer
Mon. MW   7.8 kDa
Sequence   FASTA