Histatin 3

Anthony Giannetti.

SASDYK2 – Histatin 3 peptide

Histatin-3
MWexperimental 4 kDa
MWexpected 4 kDa
VPorod 7 nm3
log I(s) 2.86×101 2.86×100 2.86×10-1 2.86×10-2
Histatin-3 small angle scattering data  s, nm-1
ln I(s)
Histatin-3 Guinier plot ln 2.86×101 Rg: 1.5 nm 0 (1.5 nm)-2 s2
(sRg)2I(s)/I(0)
Histatin-3 Kratky plot 1.104 0 √3 sRg
p(r)
Histatin-3 pair distance distribution function Rg: 1.6 nm 0 Dmax: 6.6 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Histatin-3 DAMMIF model
log I(s)
 s, nm-1
Synchrotron SAXS data from solutions of Histatin 3 peptide in 20 mM HEPES, 150 mM NaCl, pH 7.4 were collected on the BL4-2 beam line at the Stanford Synchrotron Radiation Lightsource (SSRL) storage ring (Menlo Park, CA, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 1.2 m and at a wavelength of λ = 0.1127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 10.00 mg/ml was measured at 15°C. 16 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Histatin-3 (His3)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   4.1 kDa
 
UniProt   P15516 (20-51)
Sequence   FASTA