La protein binding to telomerase RNA supports an evolutionary relationship between plant and ciliate telomerase pathways.

Jenner L, Pruchkouski D, Štefanovie B, Nováková O, Kubíčková M, Fajkus P, Brázdová M, Paleček J, Sýkorová E, Nucleic Acids Res 54(5) (2026) Europe PMC

SASDYP2 – Protein La 1 - full length

La protein 1
MWexperimental 94 kDa
MWexpected 48 kDa
VPorod 111 nm3
log I(s) 1.35×10-1 1.35×10-2 1.35×10-3 1.35×10-4
La protein 1 small angle scattering data  s, nm-1
ln I(s)
La protein 1 Guinier plot ln 1.36×10-1 Rg: 5.0 nm 0 (5.0 nm)-2 s2
(sRg)2I(s)/I(0)
La protein 1 Kratky plot 1.104 0 √3 sRg
p(r)
La protein 1 pair distance distribution function Rg: 4.5 nm 0 Dmax: 13.3 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Protein La 1 - full length Rg histogram Rg, nm
La protein 1 EOM/RANCH model
La protein 1 EOM/RANCH model
La protein 1 EOM/RANCH model
La protein 1 EOM/RANCH model
SAXS data from solutions of Protein La 1 - full length in 100 mM Tris-HCl, 500 mM NaCl, pH 8 were collected on the Rigaku BioSAXS-2000 instrument (CEITEC, Brno, Czech Republic) using a Rigaku HyPix-3000 detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 1.54 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.00 mg/ml was measured at 9°C. 12 successive 300 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

La protein 1
Mol. type   Protein
Organism   Arabidopsis thaliana
Olig. state   Monomer
Mon. MW   48.1 kDa
 
UniProt   Q93ZV7-1 (1-433)
Sequence   FASTA