STI1 domain engages transient helices to mediate Dsk2 phase separation and proteasome condensation.

Acharya N, Daniel EA, Dao TP, Niblo JK, Mulvey EO, Sukenik S, Kraut DA, Roelofs J, Castañeda CA, EMBO J (2026) Europe PMC

SASDYT2 – Full-length yeast shuttle protein Dsk2

Ubiquitin domain-containing protein DSK2
MWexperimental 40 kDa
MWexpected 39 kDa
log I(s) 6.43×101 6.43×100 6.43×10-1 6.43×10-2
Ubiquitin domain-containing protein DSK2 small angle scattering data  s, nm-1
ln I(s)
Ubiquitin domain-containing protein DSK2 Guinier plot ln 6.43×101 Rg: 3.8 nm 0 (3.8 nm)-2 s2
(sRg)2I(s)/I(0)
Ubiquitin domain-containing protein DSK2 Kratky plot 1.104 0 √3 sRg

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Full-length yeast shuttle protein Dsk2 in 20 mM NaPhos, 0.5 mM EDTA, 0.02% NaN3, pH 6.8 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS) storage ring (Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 300.00 μl sample at 6.1 mg/ml was injected at a 0.65 ml/min flow rate onto a Shodex KW-800 series column at 25°C. 600 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Ubiquitin domain-containing protein DSK2 (DSK2)
Mol. type   Protein
Organism   Saccharomyces cerevisiae
Olig. state   Monomer
Mon. MW   39.3 kDa
 
UniProt   P48510 (1-373)
Sequence   FASTA