SASDYT6 – Full-length Ras GTPase-activating protein-binding protein 1, G3BP1 (residues 1-466) at pH 6.0, 150 mM NaCl

Synchrotron SAXS data from solutions of G3BP1 in 50mM MES pH 6, 150mM NaCl, 1mM DTT, 1% glycerol, were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 60.00 μl sample at 8.5 mg/ml was injected at a 0.75 ml/min flow rate onto a GE Superdex 200 Increase 5/150 column at 25°C. 22 successive 1 second frames were collected through the SEC elution peak of the sample. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.