SASDYU3 – N-terminal truncation mutant, of the conjugative protein of the F plasmid Type IV Secretion System, TraW.

Synchrotron SAXS data from solutions of a N-terminal truncation mutant of TraW in 20 mM HEPES, 100 mM NaCl, 5% glycerol, pH 7 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS; Argonne National Laboratory USA) using a Eiger2 XE 9M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 350.00 μl sample at 13 mg/ml was injected at a 0.5 ml/min flow rate onto a GE Superdex 75 Increase 10/300 column at 22°C. 3175 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.