SAXS
data from solutions of
Chimeric constructs - CC_1 (AtCel8A_Jo:In-AtCel9R)
in
25 mM Tris, 150 mM NaCl, pH 8
were collected
on the
XEUSS 2.0 instrument (Laboratoire de Génie Chimique, Toulouse, France)
using a Pilatus 1M detector (DECTRIS, Switzerland) detector
at a sample-detector distance of 1.3 m and
at a wavelength of λ = 0.154 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 0.9 and 12.5 mg/ml were measured
at 20°C.
12 successive
600 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
s, nm-1
