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Synchrotron SAXS
data from solutions of
Conjugative protein of the F plasmid Type IV Secretion System, TraW.
in
20 mM HEPES, 100 mM NaCl, 5% glycerol, pH 7
were collected
on the
BioCAT 18ID beam line
at the Advanced Photon Source (APS), Argonne National Laboratory storage ring
(Lemont, IL, USA)
using a Eiger2 XE 9M detector
at a sample-detector distance of 3.7 m and
at a wavelength of λ = 0.1033 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 300.00 μl sample
at 7 mg/ml was injected at a 0.60 ml/min flow rate
onto a GE Superdex 200 Increase 10/300 column
at 20°C.
2582 successive
0.500 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Synchrotron SAXS data from solutions of TraW in 20 mM HEPES, 100 mM NaCl, 5% glycerol, pH 7 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS; Argonne National Laboratory USA) using a Eiger2 XE 9M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 300.00 μl sample at 7 mg/ml was injected at a 0.6 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 2582 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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s, nm-1
