ACTN2 HCM-linked Missense Variants

Maya Noureddine.

SASDZ35 – ACTN2-R93Q at 60 degrees (°C) using batch-mode SAXS

Alpha-actinin-2
MWexperimental 945 kDa
MWexpected 208 kDa
log I(s) 4.22×10-1 4.22×10-2 4.22×10-3 4.22×10-4
Alpha-actinin-2 small angle scattering data  s, nm-1
ln I(s)
Alpha-actinin-2 Guinier plot ln 4.22×10-1 Rg: 14.0 nm 0 (14.0 nm)-2 s2
(sRg)2I(s)/I(0)
Alpha-actinin-2 Kratky plot 1.104 0 √3 sRg
p(r)
Alpha-actinin-2 pair distance distribution function Rg: 15.8 nm 0 Dmax: 49.3 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of ACTN2-R93Q at 60 degrees (°C) using batch-mode SAXS in 20 mM HEPES, 150 mM NaCl, pH 8 were collected on the B21 beam line at the Diamond Light Source storage ring (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.94 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 60°C. 25 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

ACTN2 with R93Q missense variant, dimer peak using batch-mode SAXS at 60 degrees (°C)

Alpha-actinin-2 (ACTN2)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   103.9 kDa
 
UniProt   P35609
Sequence   FASTA