| MWexperimental | 30 | kDa |
| MWexpected | 27 | kDa |
|
log I(s)
3.02×101
3.02×100
3.02×10-1
3.02×10-2
|
s, nm-1
|
Use of biomolecular emulator for characterizing flexible proteins by small-angle x-ray scattering.
Haitin Y, Manori B, Giladi M Protein Sci 35(6):e70646 (2026) Europe PMCSASDZE3 – Chloride intracellular channel protein 5
Chloride intracellular channel protein 5|
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Fits and models
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Rg, nm
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Synchrotron SAXS
data from solutions of
Chloride intracellular channel protein 5
in
20 mM HEPES, 150 mM NaCl, 10 mM beta-mercaptoethanol, pH 7.5
were collected
on the
BM29 beam line
at the ESRF storage ring
(Grenoble, France)
using a Pilatus 1M detector
at a sample-detector distance of 2.9 m and
at a wavelength of λ = 0.1 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 2 and 4 mg/ml were measured
at 5°C.
10 successive
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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