ACTN2 HCM-linked Missense Variants

Maya Noureddine.

SASDZE5 – ACTN2-R457C using SEC-SAXS (aggregate peak)

Alpha-actinin-2
MWexperimental 683 kDa
MWexpected 208 kDa
log I(s) 4.53×10-2 4.53×10-3 4.53×10-4 4.53×10-5
Alpha-actinin-2 small angle scattering data  s, nm-1
ln I(s)
Alpha-actinin-2 Guinier plot ln 4.54×10-2 Rg: 12.8 nm 0 (12.8 nm)-2 s2
(sRg)2I(s)/I(0)
Alpha-actinin-2 Kratky plot 1.104 0 √3 sRg
p(r)
Alpha-actinin-2 pair distance distribution function Rg: 15.9 nm 0 Dmax: 49.9 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of ACTN2-R457C using SEC-SAXS (aggregate peak) in 20 mM HEPES, 150 mM NaCl, pH 8 were collected on the B21 beam line at the Diamond Light Source storage ring (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.94 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 4°C. 600 successive 3.200 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

ACTN2 with R457C missense variant using SEC-SAXS with aggregate peak selected

Alpha-actinin-2 (ACTN2)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   103.9 kDa
 
UniProt   P35609
Sequence   FASTA