Use of biomolecular emulator for characterizing flexible proteins by small-angle x-ray scattering.

Haitin Y, Manori B, Giladi M Protein Sci 35(6):e70646 (2026) Europe PMC

SASDZF3 – Chloride intracellular channel protein 5, F34D mutant

Chloride intracellular channel protein 5
MWexperimental 28 kDa
MWexpected 27 kDa
log I(s) 9.26×101 9.26×100 9.26×10-1 9.26×10-2
Chloride intracellular channel protein 5 small angle scattering data  s, nm-1
ln I(s)
Chloride intracellular channel protein 5 Guinier plot ln 9.26×101 Rg: 2.1 nm 0 (2.1 nm)-2 s2
(sRg)2I(s)/I(0)
Chloride intracellular channel protein 5 Kratky plot 1.104 0 √3 sRg

Data validation

Fits and models

log I(s)
 s, nm-1
Chloride intracellular channel protein 5, F34D mutant Rg histogram Rg, nm
Chloride intracellular channel protein 5 OTHER model
Chloride intracellular channel protein 5 OTHER model
Chloride intracellular channel protein 5 OTHER model
Chloride intracellular channel protein 5 OTHER model
Synchrotron SAXS data from solutions of Chloride intracellular channel protein 5, F34D mutant in 20 mM HEPES, 150 mM NaCl, 10 mM beta-mercaptoethanol, pH 7.5 were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.0992 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 2 and 4 mg/ml were measured at 5°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Chloride intracellular channel protein 5 (CLIC5)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   26.9 kDa
 
UniProt   Q9NZA1 (175-410)
Sequence   FASTA