Plasmodium circumsporozoite proteins

Yann Sterckx.

SASDZG3 – Plasmodium falciparum Circumsporozoite protein (CSP) full-length

Circumsporozoite protein
MWexperimental 40 kDa
MWexpected 41 kDa
VPorod 70 nm3
log I(s) 2.61×102 2.61×101 2.61×100 2.61×10-1
Circumsporozoite protein small angle scattering data  s, nm-1
ln I(s)
Circumsporozoite protein Guinier plot ln 2.62×102 Rg: 5.0 nm 0 (5.0 nm)-2 s2
(sRg)2I(s)/I(0)
Circumsporozoite protein Kratky plot 1.104 0 √3 sRg
p(r)
Circumsporozoite protein pair distance distribution function Rg: 5.4 nm 0 Dmax: 23.9 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Circumsporozoite protein BILBOMD model
Synchrotron SAXS data from solutions of Plasmodium falciparum Circumsporozoite protein (CSP) full-length in 50 mM Tris, 150 mM NaCl, pH 7.5 were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 70.00 μl sample at 10 mg/ml was injected at a 0.08 ml/min flow rate onto a GE Superdex 200 Increase 3.2/300 column at 20°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

X-ray Exposure time = UNKNOWN. Number of frames = UNKNOWN

Circumsporozoite protein (PfCSPFL)
Mol. type   Protein
Organism   Plasmodium falciparum (isolate NF54)
Olig. state   Monomer
Mon. MW   41.0 kDa
 
UniProt   P19597 (20-384)
Sequence   FASTA