Plasmodium circumsporozoite proteins

Yann Sterckx.

SASDZJ3 – Plasmodium berghei Circumsporozoite Protein (CSP) full-length

Circumsporozoite protein
MWexperimental 37 kDa
MWexpected 35 kDa
VPorod 134 nm3
log I(s) 2.50×10-2 2.50×10-3 2.50×10-4 2.50×10-5
Circumsporozoite protein small angle scattering data  s, nm-1
ln I(s)
Circumsporozoite protein Guinier plot ln 2.51×10-2 Rg: 5.4 nm 0 (5.4 nm)-2 s2
(sRg)2I(s)/I(0)
Circumsporozoite protein Kratky plot 1.104 0 √3 sRg
p(r)
Circumsporozoite protein pair distance distribution function Rg: 5.8 nm 0 Dmax: 25.3 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Circumsporozoite protein BILBOMD model
Synchrotron SAXS data from solutions of Plasmodium berghei Circumsporozoite Protein (CSP) full-length in 50 mM Tris, 500 mM NaCl, pH 7.5 were collected on the SWING beam line at the SOLEIL storage ring (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 10 mg/ml was injected at a 0.25 ml/min flow rate onto a Shodex KW404-4F column at 20°C. 750 successive 0.990 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Circumsporozoite protein (PbCSPFL)
Mol. type   Protein
Organism   Plasmodium berghei
Olig. state   Monomer
Mon. MW   35.4 kDa
 
UniProt   P06915 (21-327)
Sequence   FASTA